The effect of guanidine hydrochloride on crystalline pepsin.

Effective Parameters on the Partition Coefficient of Guanidine Hydrochloride in the Poly (Ethylene Glycol) +Phosphate +Water System at 298.15 K

Liquid-liquid equilibrium data prediction using large margin nearest neighbor

Guanidine hydrochloride has been widely used in the initial recovery steps of active protein from the inclusion bodies in aqueous two-phase system (ATPS). The knowledge of the guanidine hydrochloride effects on the liquid-liquid equilibrium (LLE) phase diagram behavior is still inadequate and no comprehensive theory exists for the prediction of the experimental trends. Therefore the effect the ...

Guanidine hydrochloride: An efficient catalyst for the synthesis of 2-hydrazolyl-4-thiazolidinone derivatives under solvent free conditions

A Highly efficient protocol has been developed for the synthesis of 2-hydrazolyl-4-thiazolidinone derivatives installing a one pot three component coupling reaction of an aromatic aldehyde, thiosemicarbazide and maleic anhydride using guanidine hydrochloride as highly inexpensive and environmentally friendly catalyst under solvent free condition at 1200C with good to excellent yields, It offers...

Comparative Study Between Different Methods of Collagen Extraction from Fish and its Properties

Collagen is the most abundant protein of animal origin, comprising approximately 30% of total animal protein. There are at least 19 variants of collagen, named type I to XIX. Types I, II, III and V are the fibrous collagens. Type I collagen is found in all connective tissue, including bones and skins. It is a heteropolymer of two "1 chains and one" 2 chain. It consists of one-third glycine, con...

Improvement of Leishmania major Amastig~te ELISA Test Using Various Antigen-Antibody Dissociating Agents and Pepsin Digestion

Amastigotes arc main pathogenic stage of I.eishmania parasite in mammalian host. During the course of leishmania infection the amastigotc surface would be covered by host proteins like immunoglobulins and complement components. These proteins produce false positive reaction and low signal/noise ratio in evaluation of antibody raising against lesion-dcrived amastigotc by indirect ELISA. 1'0 over...